Modulation of Glycosaminoglycan Levels in Tree Shrew Sclera during Lens-Induced Myopia Development and Recovery

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Modulation of glycosaminoglycan levels in tree shrew sclera during lens-induced myopia development and recovery.

PURPOSE In juvenile tree shrews, positioning a negative-power lens in front of an eye produces a hyperopic shift in refractive state and causes a compensatory increase in axial length over several days so that the eye is myopic when the lens is removed. During negative lens compensation, the scleral extracellular matrix is remodeled. A biomechanical property of the sclera, creep rate, increases...

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Alterations in protein expression in tree shrew sclera during development of lens-induced myopia and recovery.

PURPOSE During the development of, and recovery from, negative lens-induced myopia there is regulated remodeling of the scleral extracellular matrix (ECM) that controls the extensibility of the sclera. Difference gel electrophoresis (DIGE) was used to identify and categorize proteins whose levels are altered in this process. METHODS Two groups of five tree shrews started monocular lens wear 2...

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Differential protein expression in Tree Shrew sclera during development of lens-induced myopia and recovery

PURPOSE The tree shrew model of refractive development is particularly useful because, like humans, tree shrews have a fibrous sclera. Selective changes in some candidate extracellular matrix proteins and mRNAs have been found in the sclera during the development of and recovery from induced myopia. We undertook a more neutral proteomic analysis using two-dimensional gel electrophoresis and mas...

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Steady state mRNA levels in tree shrew sclera with form-deprivation myopia and during recovery.

PURPOSE In tree shrews, visual form deprivation induces myopia and tissue remodeling in the sclera, characterized by decreased levels of collagen and glycosaminoglycans (GAGs) and increased levels of matrix metalloproteinases (MMPs). Removal of the visual deprivation allows recovery. This study investigated whether these changes are accompanied by changes in steady state mRNA levels in the scle...

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The time course of changes in mRNA levels in tree shrew sclera during induced myopia and recovery.

PURPOSE In tree shrews, visual form deprivation produces increased axial elongation of the deprived eye and a myopic shift in refractive state. A change in scleral extensibility (creep rate) is closely associated with the change in axial elongation rate. These effects may be due to scleral tissue remodeling produced by a change in scleral gene expression. In this study, the authors investigated...

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ژورنال

عنوان ژورنال: Investigative Opthalmology & Visual Science

سال: 2007

ISSN: 1552-5783

DOI: 10.1167/iovs.06-0906